ATP-polyamine-biotin
规格
| Cas Number | 1800401-93-7 |
| 规格或纯度 | ≥99% |
| 纯度 | ≥99% |
| 包装 | 1mg 或 5mg 或 2mg |
产品信息
| 品牌 | 阿拉丁 |
| 溶解性 | H2O : 6 mg/mL (6.34 mM; Need ultrasonic and warming) |
| 过滤标签 | Biochemical Assay Reagents |
| 储存温度 | 避光,-80℃储存 |
| 运输条件 | 超低温冰袋运输 |
| 生化和生理学机理 | ATP 多胺生物素是第一种可渗透细胞的 ATP 类似物，是一种高效的激酶共底物。ATP 多胺生物素可促进活细胞中激酶底物的生物素标记。 |
| 英文描述 |
ATP-polyamine-biotin, the first cell-permeable ATP analogue, is an efficient kinase cosubstrate. ATP-polyamine-biotin promotes biotin labeling of kinase substrates in live cells. In Vitro ATP-polyamine-biotin (APB) is incubated with PKA kinase and full-length protein substrate, myelin basic protein (MBP). Biotinylation was observed only in presence of kinase. In addition, MBP biotinylation was lost in the absence of ATP-polyamine-biotin, in presence of the kinase inhibitor staursporine or upon incubation with acid due to cleavage of phosphoramidate bond. Biotinylated kemptide product is observed only in the presence of APB cosubstrate. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Form:Solid |