BODIPY 500/510 C1, C12(脂肪酸绿色荧光探针)

阿拉丁的BODIPY 500/510 C1, C12(脂肪酸绿色荧光探针)，英文名为BODIPY 500/510 C1, C12 ，也称C1-BODIPY 500/510 C12、 BODIPY-labeled Lauric Acid、BODIPY标记月桂酸、BODIPY标记十二烷酸，是一种BODIPY荧光标记的饱和脂肪酸，可以穿过细胞膜进入细胞内部，对细胞内的极性脂类进行定位和特异性染色，常作为荧光探针用于脂肪酸摄取、脂质转运、肠道脂质吸收、脂肪或肝脏等组织脂质的吸收和累积等研究，或作为通用型膜探针对细胞膜进行染色等，也可用作各种荧光磷脂的合成前体，适用于荧光显微镜、激光共聚焦显微镜、流式细胞仪、荧光酶标仪等荧光检测系统。本产品和Thermo公司的BODIPY™ 500/510 C1, C12 (4,4-Difluoro-5-Methyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Dodecanoic Acid) 的分子式相同。BODIPY，即4,4-二氟-5-甲基-4-硼杂-3a,4a-二氮杂-s-引达省-3-月桂酸，英文名为4,4-Difluoro-5-methyl-4-bora-3a,4a-diaza-s- indacene-3-dodecanoic acid，是一种具有强紫外吸收能力的小分子染料，染料间干扰小，光谱很窄，染料分子不带电荷，呈中性，其荧光峰比较尖锐，量子产率高。与大多数长波长染料不同，BODIPY荧光基团本质上是亲脂性的，所以可以模拟天然脂质的性质。该系列染料在不同生理条件下相对稳定，对环境的极性和pH值相对不敏感，由于其结构具有非对称性，故有多种衍生结构产物，BODIPY 500/510 C1, C12即为其中一种。BODIPY 500/510 C1, C12的分子式为C₂₂H₃₁BF₂N₂O₂，分子量为404.3，在掺入活细胞时，可形成激发二聚体并发射红移荧光，最大激发波长为500nm，最大发射波长为510nm。脂类(Lipid)，又称脂质，是一类不溶于水而能溶于乙醇、乙醚、氯仿、苯等非极性有机溶剂的化合物。脂类是人体重要的营养素之一，其主要生理功能包括储存和供给能量、提供机体必需脂肪酸、构成细胞膜等膜性细胞组分、以及参与细胞的信号转导等。脂类是脂肪酸与醇脱水缩合生成的酯及其衍生物，动物体内主要包括脂肪、磷脂(Phospholipids)和类固醇等。脂质加工和运输缺陷是许多代谢性疾病的基础，脂质代谢异常也是导致肥胖、糖尿病、心血管疾病和癌变的主要因素。通过标记不同链长的脂肪酸，可视化脂肪酸的运输和代谢，可以更好地了解代谢功能1]。BODIPY 500/510 C1, C12常作为荧光探针用于脂肪酸摄取、脂质转运的研究，例如可以溶解在橄榄油中用于小鼠等检测肠道对于脂肪酸的吸收，检测脂肪、肝脏等对于灌胃后脂肪酸的吸收情况，研究血管平滑肌中脂质运输情况，以判断脂毒性在动脉粥样硬化发展中的作用等2]。阿拉丁BODIPY 500/510 C1, C12对细胞脂质染色的效果请参考图1。图1. 阿拉丁BODIPY 500/510 C1, C12(脂肪酸绿色荧光探针) 对NRK-52细胞染色的效果图。图A为正常NRK-52细胞在明场状态下的形态，图B显示BODIPY 500/510 C1, C12标记正常细胞膜，且正常细胞内无明显的脂滴凝聚现象；使用油酸可以诱导脂滴的产生，如图E所示经油酸处理的细胞内出现明显的脂滴凝聚，图F显示BODIPY 500/510 C1, C12可有效标记脂滴。其中细胞核使用DAPI 染色。实际染色效果会因实验条件、检测仪器等的不同而存在差异，图中效果仅供参考。BODIPY 500/510 C1, C12的建议起始工作浓度为1-10µM，小鼠灌胃的推荐剂量为0.5μg/g体重。用于细胞染色时，推荐使用DMSO作为溶剂，而用于小鼠等动物的灌胃时推荐使用橄榄油作为溶剂。

注意事项：

Aladdin's BODIPY 500/510 C1, C12 (Green-fluorescent Fatty Acid Probe) is a fluorescence-labelled fatty acid. It is cell membrane permeable and can specifically stain polar lipids in cells. It is often used as a fluorescent probe to stain cell membranes or to investigate fatty acid uptake, lipid transport, intestinal lipid uptake, lipid uptake and their accumulation in tissues such as fat and liver. It can also be used as a precursor for the synthesis of a variety of fluorescent phospholipids. Its fluorescence can be examined by fluorescence microscopy, confocal microscopy, flow cytometry, and fluorescence plate reader, etc.BODIPY (4,4-Difluoro-5-methyl-4-bora-3a,4a-diaza-s-indacene-3-dodecanoic acid) is a uncharged and neutral small molecule dye with strong UV absorption, low inter-dye interference, narrow spectral bandwidth, relatively sharp fluorescence peaks, and high fluorescence quantum yield. Unlike most other long-wavelength dyes, BODIPY is lipophilic and can mimic the properties of natural lipids. It is relatively stable under different physiological conditions and insensitive to the polarity and pH of the environment. Due to its asymmetric structure, there are a variety of derived structural products, of which BODIPY 500/510 C1, C12 is one. The molecular formula of BODIPY 500/510 C1, C12 is C22H31BF2N2O2 with a molecular weight of 404.3. When doped into living cells, it can form excimers and emit red-shifted fluorescence, with the excitation and emission maxima at 500nm and 510nm, respectively.Lipids are a group of compounds that are insoluble in water but soluble in organic solvents such as ethanol, ether, and benzene. In animals, lipids mainly include fats, phospholipids, and steroids. Defects in lipid processing and transport are the basis of many metabolic diseases, and abnormalities in lipid metabolism are also major factors leading to obesity, diabetes, cardiovascular diseases, and carcinogenesis. By labeling fatty acids with different chain lengths, the transport and metabolism of fatty acids can be visualized, which can provide better understanding of metabolic functions [1]. BODIPY 500/510 C1, C12 is commonly used as a fluorescent probe for studying fatty acid uptake and lipid transport. For example, it can be dissolved in olive oil to detect the absorption of fatty acids in the intestine of mice, assess the absorption of fatty acids in liver and adipose tissues after gavage, and investigate lipid transport in vascular smooth muscles to evaluate the role of lipotoxicity in the development of atherosclerosis [2].Please refer to Figure 1 for the performance of this product in staining cellular lipids.Figure 1. NRK-52 cells stained with Aladdin's BODIPY 500/510 C1, C12 (Green-fluorescent Fatty Acid Probe) . A. Morphology of normal NRK-52 cells under brightfield; B. Labeling of normal cell membrane with BODIPY 500/510 C1, C12, and no obvious lipid droplet aggregation was observed within the normal cells; E. NRK-52 cells treated with oleic acid, with significant aggregation of lipid droplets in cells; F. Lipid droplets labeled with BODIPY 500/510 C1, C12. C, D. Cell nuclei stained with DAPI . This figure is for reference only, which may vary due to different experimental conditions.The recommended starting working concentration for BODIPY 500/510 C1, C12 is 1-10µM. The recommended dosage for gavage in mice is 0.5µg/g body weight. For cell staining, we recommend using DMSO as the solvent, but for gavage in animals such as mice, Olive Oil is recommended.

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