GSK-3484862
规格
| Cas Number | 2170136-65-7(DMSO) |
| 规格或纯度 | 10mM in DMSO |
| 包装 | 1ml |
产品信息
| 品牌 | 阿拉丁 |
| 浓度 | 10mM in DMSO |
| 过滤标签 | 表观遗传学,DNA Methyltransferase,Compound libraries |
| 储存温度 | -80℃储存 |
| 运输条件 | 超低温冰袋运输 |
| 生化和生理学机理 | GSK-3484862 是 DNA 甲基转移酶 (Dnmt1) 的非共价抑制剂。GSK-3484862 可诱导 DNA 低甲基化以对抗癌症 .GSK-3484862 在小鼠胚胎干细胞中介导了显著的去甲基化作用，且非特异性毒性极低。 |
| 英文描述 |
GSK-3484862 is a non-covalent inhibitor for DNA methyltransferase (Dnmt1) . GSK-3484862 induces DNA hypomethylation to against cancer . GSK-3484862 mediates dramatic demethylation in murine embryonic stem cells with minimal non-specific toxicity In Vitro GSK-3484862 (0-10 µM, 6 or 14 days) induces dramatic DNA methylation loss. GSK-3484862 (0-10 µM, 4 days) results in a modest reduction in DNMT1 protein level. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Cell Viability AssayCell Line: Murine embryonic stem cells (mESC, wild-type (WT) or Dnmt1/3a/3b triple knockout (TKO)) Concentration: 2 µM and 10 µM Incubation Time: 6 or 14 days Result: Induced dramatic DNA methylation loss, with global CpG methylation levels falling from near 70% in WT mESC to less than 18% after 6 days. Western Blot AnalysisCell Line: Murine embryonic stem cells (mESC, wild-type (WT) or Dnmt1/3a/3b triple knockout (TKO)) Concentration: 2 µM and 10 µM Incubation Time: 4 days Result: Resulted in a modest reduction in DNMT1 protein level. IC50& Target:DNMT1 |