氰基硼氢化物偶联缓冲液

Description

Component：0.02 M sodium phosphate, pH 7.5, containing 0.2 M sodium chloride and 3.0 g/L sodium cyanoborohydrideCyanoborohydride Coupling Buffer is used in affinity chromatography, protein chromatography, activated/functionalized matrices and synthetic reagents. Cyanoborohydride has been used to inform a safe and effective gene-transfer system targeting hepatocytes as well as to develop a method for targeted delivery of anticancer therapeutics to cancer cells in hypoxic areas.A ready-to-use reagent used to couple amine ligands to aldehyde functional groups. The coupling buffer reaction is a reductive amination of the intermediate Schiff′s base to a stable C−N bond.Cyanoborohydride Coupling Buffer has been used:in coupling reactions between amines and glutaraldehydeto reduce hydrazone bond to a stable hydrazide bondas a component in oligonucleotide reaction mixture for coverslips functionalization