(5Z,8Z,11Z,14Z)-5,8,11,14-二十碳四烯酸 2-氧代-2H-1-苯并吡喃-7-基酯
规格
| Cas Number | 161180-11-6 |
| 规格或纯度 | ≥95% |
| 纯度 | ≥95% |
| 包装 | 100mg 或 50mg 或 10mg 或 5mg 或 25mg |
产品信息
| 别名 | 7-羟基香豆素基花生四烯酸 |
| 品牌 | 阿拉丁 |
| 溶解性 | Soluble in DMSO (50 mg/ml), ethanol (35 mg/ml), ethanol:PBS (pH 7.2)(1:1) (~50 μg/ml), DMF, and water (50 μg/ml) at 25° C. |
| 简短描述 | PLA2荧光底物 |
| 英文简短描述 | a PLA2 fluorogenic substrate |
| 过滤标签 | Phospholipase,MAGL,Metabolic Enzyme/Protease |
| 储存温度 | -20°C储存 |
| 运输条件 | 超低温冰袋运输 |
| 生化和生理学机理 | Monoacylglycerol lipase (MAGL) protein facilitates the hydrolysis of Umbelliferyl Arachidonate and generates arachidonic acid and the highly fluorescent 7-hydroxyl coumarin (7-HC). Hence, it acts as a marker in a fluorescence-based assay. |
| 描述 |
7-Hydroxycoumarinyl arachidonate (7-HCA) 是胞质磷脂酶 A2 (PLA2) 的荧光底物，也是单酰基甘油脂肪酶 (MAGL) 的荧光底物。MAGL 蛋白催化 7-Hydroxycoumarinyl arachidonate 的水解，生成花生四烯酸 (AA；HY-109590) 和高荧光的 7-羟基香豆素 (7-HC；HY-N0573)，7-HC 的释放可以用荧光计进行测量。 |
| 英文描述 |
7-Hydroxycoumarinyl arachidonate (7-HCA) is a fluorogenic substrate of cytosolic phospholipase A2 (PLA2). 7-Hydroxycoumarinyl arachidonate is also a fluorogenic substrate for monoacylglycerol lipase (MAGL). MAGL protein catalyzes the hydrolysis of 7-Hydroxycoumarinyl arachidonat to generate Arachidonic acid (AA) and the highly fluorescent 7-hydroxyl coumarin (7-HC; HY-N0573). Release of 7-HC can be measured using a fluorometer. Application： Umbelliferyl Arachidonate also known as 7-Hydroxycoumarinyl Arachidonate was used as a fluorogenic substrate for developing a fluorescence-based assay to evaluate the inhibitors against human recombinant monoacylglycerol lipase. |