AF 700 NHS Ester

AF 700 是一种明亮且具有光稳定性的近红外染料，可使用工作光谱范围为 675–700 nm 的氙弧灯、远红二极管激光器或染料泵浦激光器进行激发。AF 700 染料用于成像和流式细胞分析中稳定信号的生成，具有水溶性和 pH 值不敏感性（pH 值 4 至 pH 值 10）。该长波长 AF 染料的荧光人眼不可见，但很容易被大多数成像系统检测到。除反应性染料制剂外，我们还提供可与多种抗体、肽、蛋白、示踪剂和扩增底物偶联并且针对细胞标记和检测进行优化的 AF 700 染料，并针对细胞标记和检测进行了优化。
AF700 的NHS酯（或琥珀酰亚胺酯）是将该染料与蛋白质或抗体结合的最常用工具。NHS 酯可用于标记蛋白质、胺修饰寡核苷酸和其他含胺分子的伯胺 (R-NH2)。所得AF偶联物将比其他光谱相似的荧光团偶联物表现出更亮的荧光和更高的光稳定性
荧光基团标记：AF 700 染料
反应性基团：NHS 酯
反应性：蛋白和配体、胺修饰的寡核苷酸上的伯胺
偶联物的 Ex/Em：702/723 nm
消光系数：205,000 cm-1M-1

AF 700 is a bright and photostable near-IR dye that can be excited with a xenon-arc lamp, far-red diode lasers, or dye pumped lasers operating in the 675–700 nm range. Used for stable signal generation in imaging and flow cytometry, AF 700 dye is water soluble and pH-insensitive from pH 4 to pH 10. Fluorescence of this long-wavelength AF dye is not visible to the human eye but is readily detected by most imaging systems. In addition to reactive dye formulations, we offer AF 700 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling and detection.The NHS ester (or succinimidyl ester) of AF 700 is the most popular tool for conjugating this dye to a protein or antibody. NHS esters can be used to label to the primary amines (R-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting Alexa Fluor™ conjugate will exhibit brighter fluorescence and greater photostability than the conjugates of other spectrally similar fluorophores.
Fluorophore label: AF 700 dye
Reactive group: NHS ester
Reactivity: Primary amines on proteins and ligands, amine-modified oligonucleotides
Ex/Em of the conjugate: 702/723 nm
Extinction coefficient: 205,000 cm-1M-1
Molecular weight: ∼1400