KP372-1
规格
| Cas Number | 1374996-60-7 |
| 规格或纯度 | ≥99% |
| 纯度 | ≥99% |
| 包装 | 5mg |
产品信息
| 品牌 | 阿拉丁 |
| 溶解性 | DMSO : 17.86 mg/mL (39.83 mM; Need ultrasonic) |
| 过滤标签 | NF-κB,Akt,Reactive Oxygen Species,Metabolic Enzyme/Protease,Immunology/Inflammation,PI3K/Akt/mTOR |
| 储存温度 | -20°C储存 |
| 运输条件 | 超低温冰袋运输 |
| 生化和生理学机理 | KP372-1 是一种 Akt 抑制剂，可抑制细胞增殖，诱导细胞凋亡和厌氧反应。KP372-1 还是一种 NQO1 氧化还原循环剂，可通过产生 ROS 造成 DNA 损伤（包括 DNA 断裂）。KP372-1 可用于癌症研究（如头颈部癌症）。 |
| 英文描述 |
KP372-1 is an Akt inhibitor that inhibits proliferation and induces apoptosis and anoikis. KP372-1 is also an NQO1 redox cycling agent that causes DNA damage (including DNA breakage) by generating ROS. KP372-1 can be used in cancer research (such as head and neck squamous cell carcinoma (HNSCC) and pancreatic cancer) . In Vitro KP372-1 (0.0625, 0.125, 0.25, 0.5, 1.0 µM; 48 h) inhibits growth of JMARc42 and Tu167c2 cells with IC 50 s of 200 and 100 nM, respectively. KP372-1 (125 nM; 24 h) induces Tu167c2 cells apoptosis and induces anoikis in the JMARc42 cells. KP372-1 (125 nM; 30 min) blocks Akt, thereby decreasing the phosphorylation of the S6 ribosomal protein in both Tu167 and JMAR cells. KP372-1 (0.250, 0.5, 1.0 µM; 30 min) inhibits Akt kinase activity with an IC 50 of 250 nM in JMAR cells. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Cell Proliferation AssayCell Line: JMARc42 and Tu167c2 cells Concentration: 0.0625, 0.125, 0.25, 0.5, 1.0 µM Incubation Time: 48 h Result: Showed antiproliferative activity. Apoptosis AnalysisCell Line: Tu167c2 and JMARc42 cells Concentration: 125 nM Incubation Time: 24 h Result: Induced approximately 90% of cells apoptosis. Western Blot AnalysisCell Line: Tu167 and JMAR cells Concentration: 125 nM Incubation Time: 30 min Result: Induced a small but consistent decrease in Akt phosphorylation with a concomitant marked decrease in S6 phosphorylation. Inhibited the EGF induced phosphorylation of Aktser473 in Tu167 and AktThr308 in JMAR. Western Blot AnalysisCell Line: JMAR cells Concentration: 0.250, 0.5, 1.0 µM Incubation Time: 30 min Result: Significantly blocked Akt kinase activity in a dose-dependent fashion, with an IC 50 of 250 nM. In Vivo KP372-1(10, 20 mg/kg; i.v.; single daily for 33 days) induces NADH oxidation and impairs tumor growth in vivo without apparent toxicity. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Animal Model: Nude mice (H1299 xenografts model). Dosage: 10, 20 mg/kg Administration: Tailvein injection; single daily for 33 days Result: Affected tumor metabolism and suppressed tumor growth. Form:Solid |