L82-G17
规格
| Cas Number | 92285-87-5 |
| 规格或纯度 | ≥99% |
| 纯度 | ≥99% |
| 包装 | 50mg 或 10mg 或 5mg 或 25mg |
产品信息
| 品牌 | 阿拉丁 |
| 溶解性 | DMSO : 125 mg/mL (472.29 mM; Need ultrasonic) |
| 过滤标签 | DNA/RNA Synthesis,Cell Cycle/DNA Damage |
| 储存温度 | -20°C储存 |
| 运输条件 | 超低温冰袋运输 |
| 生化和生理学机理 | L82-G17 是一种非竞争性 DNA 连接酶 I（Lig I）选择性抑制剂。L82-G17 可抑制连接反应的第三步--磷酸二酯键的形成。L82-G17 可用作催化活性的探针。 |
| 英文描述 |
L82-G17 is an uncompetitive DNA ligase I (Lig I)-selective inhibitor. L82-G17 inhibits the third step of the ligation reaction, phosphodiester bond formation. L82-G17can be used as a probe of the catalytic activity. In Vitro L82-G17 (200 μM, 30 min) has selective uncompetitive inhibitory effect for LigI. L82-G17 (0-100 μM) increases LigI binding to non-ligatable nicked DNA binding. L82-G17 inhibits step 3 of the ligation reaction, phosphodiester bond formation. L82-G17 (0-100 μM) inhibits DNA synthesis, cell viability and s induces DNA damage. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Cell Viability AssayCell Line: HeLa cells Concentration: 0-30 μM Incubation Time: 5 days Result: Reduced cell number by about 70% at 20 μM. Cell Proliferation AssayCell Line: CH12F3 WT and CH12F3Δ/Δ cells Concentration: 0-100 μM Incubation Time: 72 h Result: Had great effect on the proliferation and survival of the parental CH12F3 cells. Form:Solid |