Native Bovine RNP/Sm Protein
规格
| 规格或纯度 | 无载体, ≥95%(SDS-PAGE), 见COA |
| 级别 | 无载体 |
| 纯度 | ≥95%(SDS-PAGE) |
| 包装 | 1mg 或 50μg |
产品信息
| 品牌 | 阿拉丁 |
| 浓度 | 见COA |
| Cas编号 | |
| Animal free | No |
| Carrier free | Yes |
| 预测分子量 | 29-35 kDa (RNP subunits), 7-15 kDa (Sm subunits) |
| 简短描述 | Carrier Free, ≥95% (SDS-PAGE), No tag |
| 英文简短描述 | Carrier Free, ≥95% (SDS-PAGE), No tag |
| 稳定性和储存 | Store at -80℃ for 6 years. Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle. |
| 过滤标签 | RNP/Sm |
| 储存温度 | -80℃储存,避免反复冻融 |
| 运输条件 | 超低温冰袋运输 |
| Accession ID | Q2KIR1,Q32PA0,Q1RMR2 |
| 储存缓冲液 | 16 mM HEPES, pH 7.6 |
| Source | 天然 |
| 生化和生理学机理 | The U1 small molecule nuclear ribonucleoprotein (U1 snRNP) antigen is an RNP/Sm multi enzyme complex composed of three U1 specific ribonucleoproteins (RNP-A, RNP-C, RNP-68K) and different Sm nucleoproteins (B/B ', D, E, F, G). RNP/Sm complex is an extractable nuclear antigen (ENA) that targets self reactive B and T cells in various rheumatic diseases. Anti RNP antibodies bind to peptides specific for U1 snRNA (RNP-A, RNP-C, RNP-68K), while anti Sm antibodies can bind to peptides specific for U1, U2, U5, and U4/6 snRNA. High titer anti-U1 snRNP autoantibodies are one of the important criteria for diagnosing mixed connective tissue diseases (MCTD, Sharpe syndrome), with a positivity rate of up to 95% -100%, and antibody titers are correlated with disease activity. This antibody can also be detected in 30% -40% of systemic lupus erythematosus (SLE) patients, but almost always accompanied by anti Sm antibodies. Anti-U1-snRNP autoantibodies can also be found in the serum of patients with other rheumatic and immune diseases, including dermatomyositis (10% -20%), unclassified connective tissue disease (20%), Sjogren's syndrome (20%), scleroderma (7%), and overlap syndrome (27%). |
| 描述 |
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| 英文描述 |
Biochemical Test: ≥95% (SDS-PAGE); Western blot with anti-RNP/Sm autoantibody-positive sample.
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| SDS-PAGE | 29 - 35 kDa (RNP subunits) & 7 - 15 kDa (Sm subunits), under reducing conditions; 29 - 35 kDa (RNP subunits) & 7 - 15 kDa (Sm subunits), under non-reducing conditions. |