Necrostatin-34
规格
| Cas Number | 375835-43-1 |
| 规格或纯度 | ≥98% |
| 纯度 | ≥98% |
| 包装 | 100mg 或 50mg 或 10mg 或 5mg 或 25mg |
产品信息
| 品牌 | 阿拉丁 |
| 溶解性 | DMSO : 125 mg/mL (325.11 mM; Need ultrasonic) |
| 过滤标签 | Apoptosis,RIP kinase |
| 储存温度 | 避光,-80℃储存 |
| 运输条件 | 超低温冰袋运输 |
| 生化和生理学机理 | Necrostatin-34（Nec-34）是一种 RIPK1 激酶抑制剂，它通过占据激酶结构域中一个独特的结合口袋，将 RIPK1 激酶稳定在非活性构象中。 |
| 英文描述 |
Necrostatin-34 (Nec-34), a RIPK1 kinase inhibitor, stabilizes RIPK1 kinase in an inactive conformation by occupying a distinct binding pocket in the kinase domain. In Vitro Necrostatin-34 (Nec-34) exhibits IC 50 values of 667 nM and 134 nM for TNFα in FADD def-Jurkat cells and L939 cells, respectively. Necrostatin-34 (Nec-34, 10 μM) inhibits the dimerization-induced RIPK1 activation as examined by phosphorylation of Ser166 (p-S166) of RIPK1, a biomarker for RIPK1 activation. Necrostatin-34 (Nec-34) may block TNFα-induced complex II formation by inhibiting the activation of RIPK1 kinase. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Western Blot AnalysisCell Line: RIPK1 knockout L929 cells transfected with an expressing vector encoding an inducible and dimerizable RIPK1 fused with FKBP at the C-terminus. Concentration: 10 μM. Incubation Time: 30 min (and then 100 ng/mL TNFα was added for indicated periods of time). Result: Downregulated p-S166 levels. Form:Solid |