NSP-SA-NHS
规格
Cas Number | 199293-83-9 |
规格或纯度 | ≥98% |
纯度 | ≥98% |
包装 | 10mg 或 1mg 或 5mg 或 25mg |
产品信息
品牌 | 阿拉丁 |
溶解性 | DMSO : 11.36 mg/mL (16.66 mM; ultrasonic and warming and heat to 60°C) |
过滤标签 | Fluorescent Dye |
储存温度 | 避光,-20°C储存,干燥 |
运输条件 | 超低温冰袋运输 |
生化和生理学机理 | NSP-SA-NHS 是一种可用于化学发光免疫测定的吖啶酯。利用 NSP-SA-NHS 标记的双酚 A(BPA),开发了一种快速灵敏的化学发光免疫测定方法。 |
英文描述 |
NSP-SA-NHS is an acridinium ester that can be used for chemiluminescent immunoassay. A rapid and sensitive chemiluminescent immunoassay of Bisphenol A (BPA) with NSP-SA-NHS-labeled has been developed. In Vitro NSP-SA-NHS marking?\n(1) 1mg BSA was dissolved in 0.3 mL PBS (solution A), and 0.2 mg NSP-SA-NHS was dissolved in 40 μL DMF (solution B). ?\n(2) Add solution B to solution A, mix at 18°C, 130 rpm, and dark for 12 h. ?\n(3) The mixture was dialyzed with 500 mL of distilled water at 4°C for 2 d, during which distilled water was changed twice (solution C). ?\n(4) Slowly add solution B to solution C, mix at 18°C, 130 rpm, and dark for 24 h, and then dialysis with distilled water for 48 h. During this period, change distilled water four times. ?\n(5) Dilute the coupling compound with distilled water to a suitable concentration, and analyze it with an ultraviolet spectrophotometer. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Form:Solid |