Recombinant Human ALIX Protein
规格
| Cas Number | |
| 规格或纯度 | 无载体, ≥95%(SDS-PAGE), 见COA |
| 级别 | 无载体 |
| 纯度 | ≥95%(SDS-PAGE) |
| 包装 | 1mg 或 100μg 或 10μg 或 50μg |
产品信息
| 品牌 | 阿拉丁 |
| 浓度 | 见COA |
| Animal free | No |
| Carrier free | Yes |
| 预测分子量 | 19.3 kDa |
| 氨基酸 | 559-718 aa |
| Protein length | 蛋白片段 |
| 表达系统 | 大肠杆菌 |
| 蛋白标签 | N-His |
| 序列 | MHHHHHHATFISVQLKKTSEVDLAKPLVKFIQQTYPSGGEEQAQYCRAAEELSKLRRAAVGRPLDKHEGALETLLRYYDQICSIEPKFPFSENQICLTFTWKDAFDKGSLFGGSVKLALASLGYEKSCVLFNCAALASQIAAEQNLDNDEGLKIAAKHYQFASGAFLHIKETVLSALSREPTVDISPDTVGTLSLIMLAQAQEVFFLKATRDKMKDAIIAKLANQAADYFGDAFKQCQYKDTLPKEVFPVLAAKHCIMQANAEYHQSILAKQQKKFGEEIARLQHAAELIKTVASRYDEYVNVKDFSDKINRALAAAKKDNDFIYHDRVPDLKDLDPIGKATLVKSTPVNVPISQKFTDLFEKMVPVSVQQSLAAYNQRKADLVNRSIAQMREATTLA |
| 复溶(Reconstitution) | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| 简短描述 | Carrier Free, ≥95% (SDS-PAGE), E. coli, N-His, 559-718 aa |
| 英文简短描述 | Carrier Free, ≥95% (SDS-PAGE), E. coli, N-His, 559-718 aa |
| 稳定性和储存 | -20℃保存1年；复溶后建议分装，避免反复冻融。 |
| 过滤标签 | Gene ID:10015,Accession#:Q8WUM4,ALIX,PDCD6IP |
| 储存温度 | -20°C储存,避免反复冻融 |
| 运输条件 | 超低温冰袋运输 |
| Accession ID | Q8WUM4 |
| 物理形态 | 冻干(Lyophilized) |
| 储存缓冲液 | Lyophilized from 20mM Tris, 500M NaCl, 8M Urea, 250mM Imidazole, pH 8.0 |
| 内毒素浓度 | <1.0 EU/μg |
| Source | 重组表达 |
| 生化和生理学机理 | Class E VPS protein involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome. Binds to the phospholipid lysobisphosphatidic acid (LBPA) which is abundant in MVBs internal membranes. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses). Appears to be an adapter for a subset of ESCRT-III proteins, such as CHMP4, to function at distinct membranes. Required for completion of cytokinesis. Involved in HIV-1 virus budding. Can replace TSG101 it its role of supporting HIV-1 release; this function implies the interaction with CHMP4B. May play a role in the regulation of both apoptosis and cell proliferation. Post-translational: May be phosphorylated on tyrosine residues by activated PDGFRB. |
| SDS-PAGE | 17.2 kDa, under reducing conditions; 17.8 & 37.4 kDa, under non-reducing conditions. |